Device

Part:BBa_J119349:Design

Designed by: Kelly Cochran   Group: Eckdahl Lab   (2014-06-30)


Ammeline Biosensor with Riboswitch D2 + RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 742
    Illegal AgeI site found at 854
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The only differences between the M6C" riboswitch and D2 is the RBS and sequestration sequence location.


Source

Riboswitch sequence TCAACACTTCATTTAATCCCGTGTATATGGTTTCGGAGCTTCCACCAAGAGCCTTAAACTCTTGACTATGAAGTCTGTCGCTTTATCCGAAATTTTATACACAGGATTGACTATGAAG

References